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Bottle Reference Chart
 

REFERENCE CHART
HOLDING TIME, BOTTLE TYPE, PRESERVATION, MINIMUM VOLUME

Analytical Parameter Maximum Holding Time Required Container Type Required Preservative Minimum Amount
Alkalinity (Bicarb, Carb, Hyd, and Tot); SM 2320 B 14 days Plastic / Glass 0-6°C 100 mL
Ammonia (NH3 as N); SM 4500NH3 B/C or B/G 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200 mL
Asbestos (TEM) 48 hours Plastic / Glass 0-6°C 1000 mL
Bioassay (Acute Tox) % survival 36 hours Plastic cubitainer 0-6°C 1-5 gal
Biochemical Oxygen Demand (BOD); SM 5210 B 48 hours Plastic / Glass 0-6°C 250 mL
Chromium, Hexavalent (CrVI); SM 3500Cr B 24 hours Plastic / Glass 0-6°C 200 mL
Chloride (Cl-); EPA 300.0 28 days Plastic / Glass 0-6°C 100 mL
Chlorine, residual; SM 4500Cl B or G 15 minutes Field Measurement
Chemical Oxygen Demand (COD); SM 5220 D or EPA 410.4 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 50 mL
Chlorophyll A (Algal Biomass) / Pheophytin; SM10200 H 48 Hours to filter
28 days once filtered
Amber Plastic 0-6°C, unfiltered
-20°C, filtered
1 L
Coliform, Total / Fecal; SM 9221 B/E 8 hrs – wastewater
24 hrs – drinking water
Plastic (sterile) Na2S2O3 + <10°C 100 mL
Coliform, Total / E.Coli; SM 9223 B
(Present/Absent or Quantitray)
24 hours Plastic (sterile) Na2S2O3 + <10°C 100 mL
Color; SM 2120 B 48 hours Plastic / Glass 0-6°C 100 mL
Conductivity (EC/SC); SM 2510 B 28 days Plastic / Glass 0-6°C 100 mL
Cyanide Total or WAD; SM 4500CN B/C/E or B/C/I 14 days Amber Plastic NaOH pH >10+0-6°C 100 mL
Dissolved Organic Carbon (DOC); SM 5310 B Filter with 48 hours. 28 days Amber Glass VOA vial
(sample in triplicate)
Filter then add HCl to pH <2+ 0-6°C 50 mL
Dissolved Oxygen (DO); SM 4500O G 15 minutes Field Measurement
Enterococcus by Enterolert 8 hours Plastic (sterile) Na2S2O3 + <10°C 100 mL
Fluoride (F-); EPA 300.0 28 days Plastic / Glass 0-6°C 100 mL
Heterotrophic Plate Count (HPC/SPC); SM 9215 E 24 hours Plastic (sterile) Na2S2O3 + 0-6°C 100 mL
Hardness (by titration); SM 2340 C 180 days Plastic / Glass HNO3 pH <2 250 mL
Metals, Dissolved {2}; EPA 200.8 Filter within 15 minutes{2}, 180 days Plastic / Glass Filter then add HNO3 100 mL
Metals, Total; EPA 200.8 180 days Plastic / Glass HNO3 pH <2 100 mL
Mercury (Hg) EPA 245.1 / 7471 28 days Plastic / Glass HNO3 pH <2 100 mL
Mercury, Total; EPA 1631 48 hours to preserve
90 days once preserved
Glass, Dbl Bagged HCl 100 mL
Mercury, Dissolved {2}
EPA 1631
Filter within 24 hours, preserve within 48 hours
90 days once preserved
Glass, Dbl Bagged
(Zero Headspace)
Filter in lab, then HCl 100 mL
Mercury, Methyl; EPA 1630 48 hours to preserve
180 days, preserved
Glass, Dbl Bagged 0-6°C + HCl if < 10 ppth salinity {8} 100 mL
Mercury, Dissolved {2} Methyl; EPA 1630 48 hrs to filter & preserve
180 days once preserved
Glass, Dbl Bagged
(Zero Headspace)
Filter in Lab, 0-6°C + HCl if < 10 ppth salinity {8} 100 mL
Nitrate (as N or as NO3-); EPA 300.0 48 hours Plastic / Glass 0-6°C 100 mL
Nitrite (NO2 as N); SM 4500NO2 B 48 hours Plastic / Glass 0-6°C 100 mL
Nitrate+Nitrite as N (NO3+NO2-N) EPA 353.2, or SM NO3 F 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 100 mL
Nitrogen, Total Kjeldahl (TKN); SM 4500Norg + SM 4500NH3 B 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200 mL
Nitrogen, Total Organic (TON) –calc 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200 mL
Odor; SM 2150 B 6 hours (recommended) / 24 hour regulatory Glass only 0-6°C 250 mL
Oil & Grease, Total and/or Hydrocarbons EPA 1664 28 days Glass only HCl pH <2 + 0-6°C 1
{10}
L
Chlorinated Pesticides & PCBs EPA 608/8081/8082 7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6} 0-6°C {5} 1 L
Polynuclear Aromatic Hydrocarbons (PAH's) EPA 625/8270 7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6} 0-6°C {5} 1 L
Polynuclear Aromatic Hydrocarbons (PAH's) EPA 625/8270 7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6} 0-6°C {5} 1 L
Semivolatile Organics
EPA 625/8270
7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6} 0-6°C {5} 1 L
Pyrethroid Pesticides
GCMS-NCI-SIM/ 8270M / GCMS/MS
7 days /3 days {9}
14 days, soil or sediment
Glass Amber Liter {6}
Solids in Poly SJ {11}
0-6°C {5}
Solids to be Frozen
1
1
L
8oz
Carbamate Pesticides
EPA 632/8321
7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6} 0-6°C 1 L
Volatile Organics
EPA 524.2 {4}
14 days, preserved Glass VOA vial {1}
(sample in triplicate)
HCl pH <2 + 0-6°C 40 mL
Volatile Organics
EPA 624 {3}
(Includes Acrolein and Acrylonitrile)
14 days unpreserved
14 days HCl preserved to pH 2
(Benzene, Ethyl Benzene, Toluene only) {11}
3 days unpreserved for Acrolein {12}
Total of Six 40 mL VOA Vials; Two Pres w/ HCl, Two Unpreserved, Two Client preserved to pH 4-5. {1} 0-6°C
see holding time for additional preservation)
One 40mL of each container type described
Volatile Organics; EPA 8260 {3} 14 days Glass VOA vial {1}
(sample in triplicate)
HCl pH <2 + 0-6°C 40 mL
TPH Diesel/Motor Oil; EPA 8015 7 days Glass Amber Liter {6} 0-6°C 1 L
Tributyltin (TBT) 7 days (recommended) Glass Amber Liter {6} 0-6°C 1 L
TPH Gas/ BTEX/ MTBE; EPA 8260 14 days, preserved Glass VOA vial {1} HCl pH <2 + 0-6°C 40 mL
Dioxin; EPA 1613 1 year Glass Amber Liter {6} 0-6°C {5} 1 L
Perchlorate; EPA 314.0 28 days Plastic / Glass 0-6°C 100 mL
pH; SM 4500 H+ B 15 minutes Plastic / Glass 0-6°C 100 mL
Phenols, EPA 420.4 28 days Glass Amber Liter H2SO4 pH <2 +0-6°C 200 mL
Phosphate, Ortho (as P); SM 4500P E 48 hours Plastic / Glass 0-6°C 100 mL
Phosphate, Ortho, Dissolved (as P); SM 4500P E 15 minutes to filter / 48 hrs once filtered Plastic / Glass 0-6°C 100 mL
Phosphorus, Total (PO4 as P); SM 4500P B/F or B/E 28 days Plastic / Glass H2SO4 pH <2+0-6°C 100 mL
Solids, Settleable (SS); SM 2540 F 48 hours Plastic / Glass 0-6°C 1 L
Solids, Total (mg/L or %); SM 2540 B, or SM 2540 G 7 days Plastic / Glass 0-6°C 100 mL
Solids, Total Dissolved (TDS); SM 2540 C 7 days Plastic / Glass 0-6°C 100 mL
Solids, Total Suspended (TSS); SM 2540 D 7 days Plastic / Glass 0-6°C 200 mL
Solids, Volatile Suspended (VSS); SM 2540 E 7 days Plastic / Glass 0-6°C 200 mL
Sulfate (SO42-), EPA 300.0 28 days Plastic / Glass 0-6°C 100 mL
Sulfide, Dissolved; SM 4500 S2- B/D 7 days Glass 250 mL AlCl3 + NaOH +0-6°C 250 mL
Sulfide, Total; SM 4500 S2- C/D 7 days Glass 250 mL NaOH + ZnAC pH >9+ 0-6°C 250 mL
Sulfite (SO3) 48 hours Glass 250 mL EDTA + 0-6°C 250 mL
Surfactants (MBAS); SM 5540 C 48 hours Plastic / Glass 0-6°C 250 mL
Suspended Sediment Conc. (SSC); ASTM D3977 7 days Plastic / Glass 0-6°C {7} 100 mL
Total Organic Carbon (TOC); SM 5310 B 28 days Glass VOA vial (in trip) HCl pH <2 + 0-6°C 40 mL
Turbidity; SM 2130 B, or EPA 180.1 48 hours Plastic / Glass 0-6°C 100 mL
Ultraviolet Absorption (UVA at 254nm); SM 5910 B 48 hours Glass Amber 125mL 0-6°C 125 mL

{1} Volatile organic samples need to be filled in multiple VOA vials without air bubbles or headspace.
{2} Dissolved metals require field or lab filtration through 0.45-micron filter prior to preservation. 40 CFR 136.3 requires filtration within 15 minutes.
{3} Volatile organic methods EPA 624 and 8260 require dechlorination using Sodium Thiosulfate (Na2S2O3) at time of sampling if chlorine is present. (Thiosulfate dechlorination bottles are available at the laboratory upon request.) Dechlorination is to occur before transferring to the appropriate VOA.
{4} Volatile organic method EPA 524.2 requires dechlorination using ascorbic acid at time of sampling if chlorine is present. (Ascorbic acid dechlorination kits are available at the laboratory upon request.) If analyzing for THMs only, Sodium Thiosulfate (Na2S2O3) may be used, and acidification can be omitted. Otherwise, dechlorinate with Ascorbic acid, then preserve with HCl. If the sample foams vigorously upon addition of HCl, discard and collect unpreserved dechlorinated sample, and notify the laboratory as the samples must be analyzed within 24 hours of collection if they are to be analyzed for any compounds other than THMs. Method 524.2 requires a travel/trip blank with each sample set collected.
{5} If sampling from a chlorinated location, collect additional bottles with 0.008% Sodium Thiosulfate (Na2S2O3) for dechlorination.
{6} Semi Volatile Organics Amber Liters (AL) should be collected in duplicate, to insure volume for re-extraction if necessary. When possible, please collect 4 AL per method to allow the laboratory to perform matrix Quality Control (MS/MSD).
{7} Suspended Sediment Conc.(SSC) requires its own container and the entire contents are used for the analysis.
{8} Preserve with HCl if less than 10 ppth Salinity, or Preserve with H2SO4 if greater than 10 ppth Salinity.
{9} Cyhalothrin in water has a 3 day hold time in reagent water per USGS study. Permethrin in water has a 3 day hold time per Storage Stability Study by CA Dept. of Food and Agriculture.
{10} 1000 mL required for RL of 5 mg/L. 250 mL bottle recommended for samples with expected concentrations of 20 mg/L or higher.
{11} If unpreserved, must be analyzed within 7 days of sampling
{12} Hold time can be extended to 14 days if preserved to pH 4-5 at time of collection.

Note: Multiple analyses may be run from the same container, provided that the bottle type, volume and preservation are appropriate.
(Rev 06.19.17)

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