REFERENCE CHART
HOLDING TIME, BOTTLE TYPE, PRESERVATION, MINIMUM VOLUME

Analytical Parameter Maximum Holding Time Required Container Type Required Preservative Minimum Amount
Alkalinity (Bicarb, Carb, Hyd, and Tot); SM 2320 B 14 days Plastic / Glass 0-6°C 100mL
Ammonia (NH3 as N); SM 4500NH3 B/C or B/G 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200mL
Asbestos (TEM) Plastic / Glass 0-6°C 1000mL
Bioassay (Acute Tox) % survival Plastic cubitainer 0-6°C 1-5gal
Biochemical Oxygen Demand (BOD); SM 5210 B Plastic / Glass 0-6°C 250mL
Chromium, Hexavalent (CrVI); SM 3500Cr B Plastic / Glass 0-6°C 200mL
Chloride (Cl); EPA 300.0 28 days Plastic / Glass 0-6° C 100mL
Chlorine, residual; SM 4500Cl B or G Field Measurement
Chemical Oxygen Demand (COD); SM 5220 D or EPA 410.4 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 50mL
Chlorophyll A (Algal Biomass) / Pheophytin; SM10200 H

28 days once filtered

Amber Plastic 0-6°C, unfiltered
-20°C, filtered
1L
Coliform, Total / Fecal; SM 9221 B/E Plastic (sterile) Na2S2O3 + <10°C 100mL
Coliform, Total / E.Coli; SM 9223 B
(Present/Absent orQuantitray)
Plastic (sterile) Na2S2O3 + <10°C 100mL
Color; SM 2120 B Plastic / Glass 0-6°C 100mL
Conductivity (EC/SC); SM 2510 B 28 days Plastic / Glass 0-6°C 100mL
Cyanide Total or WAD; SM 4500CN B/C/E or B/C/I 14 days Amber Plastic NaOH pH >10+0-6°C 100mL
Dissolved Organic Carbon (DOC); SM 5310 B

28 days

250 mL Amber glass Filter then add HCL to pH< 2 + 0-6°C 50mL
Dissolved Oxygen (DO); SM 4500O G Field Measurement
Enterococcus by Enterolert Plastic (sterile) Na2S2O3 + <10°C 100mL
Fluoride (F-); EPA 300.0 28 days Plastic / Glass 0-6°C 100mL
Heterotrophic Plate Count (HPC/SPC); SIMPLATE Plastic (sterile) Na2S2O3 + 0-6°C 100mL
Hardness (by titration); SM 2340 C 180 days Plastic / Glass HNO3 pH <2 250mL
Metals, Dissolved {2}; EPA 200.8
6 months
Plastic / Glass Filter then add HNO3 100mL
Metals, Total; EPA 200.8
Metals, Total; EPA 6010/6020
6 months Plastic / Glass
Solids in Glass SJ
HNO3 pH <2
None
100mL80z
1
Mercury (Hg) EPA 245.1
Mercury (Hg) EPA 7471
28 days Plastic / Glass
Solids in Glass SJ
HNO3 pH <2
0-6°C
100
1
Mercury, Total; EPA 1631 (Aqueous)

Mercury, Total; EPA 1631 (Sludge or Solids)

90 days once preserved
90 days frozen

Glass, Dbl Bagged

Solids in Glass SJ

HCl

0-6°C

100mL

180z

Mercury, Dissolved {2}; EPA 1631

90 days once preserved

Glass, Dbl Bagged Filter in lab, then HCl 100mL
Mercury, Methyl; EPA 1630 (Aqueous)

Mercury, Methyl; EPA 1630 (Sludge or Solids)

6 months preserved
6 months frozen

Glass, Amber Dbl Bagged

Solids in Amber Poly SJ

0-6°C + HCl if < 10 ppth
salinity {8}
Solids to be Frozen
100mL

180z

Mercury, Dissolved {2} Methyl; EPA 1630

6 months once preserved

Glass, Amber Dbl Bagged Filter in Lab, 0-6°C + HCl if < 10 ppth salinity {8} 100mL
Nitrate (as N); EPA 300.0 Plastic / Glass 0-6°C 100mL
Nitrite (as N); SM 4500NO2 B Plastic / Glass 0-6°C 100mL
Nitrate+Nitrite as N (NO3+NO2-N) EPA 353.2, or SM NO3 F 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 100mL
Nitrogen, Total Kjeldahl (TKN); SM 4500Norg + SM 4500NH3 B 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200mL
Nitrogen, Total Organic (TON) TKN-NH3= TON calc 28 days Plastic / Glass H2SO4 pH <2 +0-6°C 200mL
Odor; SM 2150 B Glass only 0-6°C 250mL
Oil & Grease, Total and/or Hydrocarbons EPA 1664 28 days Amber Glass only HCl pH <2 + 0-6°C 1
{10}L
Chlorinated Pesticides & PCBs
EPA 608.1/8081/8082
7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6}
Solids in Glass SJ
0-6°C {5}
0-6°C
1L
180z
Polynuclear Aromatic Hydrocarbons
(PAH’s) EPA 625.1/8270
7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6}
Solids in Glass SJ
0-6°C {5}
0-6°C
1L
180z
Organophosphorus Pesticides EPA 614/8141 7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6}
Solids in Glass SJ
0-6°C {5}
0-6°C
1L
180z
Semivolatile Organics
EPA 625.1/8270
7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6}
Solids in Glass SJ
0-6°C {5}
0-6°C
1L
180z
Pyrethroid Pesticides
GCMS-NCI-SIM/ 8270M / GCMS/MS
7 days /3 days {9}
14 days, soil or sediment
Glass Amber Liter {6}
Solids-Amber Glass SJ {11}
0-6°C {5}
Solids to be Frozen
1L
180z
Carbamate Pesticides EPA 632/8321 7 days, Aqueous
14 days, Sludge or Solid
Glass Amber Liter {6}
Solids in Glass SJ
0-6°C 1L
Volatile Organics
EPA 524.2 {4}
14 days, preserved Glass VOA vial {1} (sample in triplicate) HCl pH <2 + 0-6°C 40mL
Volatile Organics EPA 624.1 {3} (Includes Acrolein and Acrylonitrile) 14 days unpreserved
14 days HCl preserved to pH 2 (Benzene, Ethyl Benzene, Toluene only) {11}
3 days unpreserved for Acrolein {12} THMS only—3 HCl VOAs
Total of Six 40 mL VOA Vials; Two Pres w/ HCl, Two Unpreserved, Two Client preserved to pH 4-5. {1} 0-6°C
(see holding time for additional preservation)
One 40mL ofeachcontainer typedescribed
Volatile Organics; EPA 8260 {3} 14 days Glass VOA vial {1}
(sample in triplicate)
HCl pH <2 + 0-6°C 40mL
TPH Diesel/Motor Oil; EPA 8015 7 days Glass Amber Liter {6} 0-6°C 1L
Tributyltin (TBT) 7 days (recommended) Glass Amber Liter {6} 0-6°C 1L
TPH Gas/ BTEX/ MTBE; EPA 8260 14 days preserved Glass VOA vial {1} HCl pH <2 + 0-6°C 40mL
Dioxin; EPA 1613 1 year Glass Amber Liter {6} 0-6°C {5} 1L
Perchlorate; EPA 314.0 28 days Plastic/Glass 0-6°C 100mL
pH; SM 4500 H+ B Plastic / Glass 0-6°C 100mL
Phenols, EPA 420.1 28 days Glass Amber 250 mL H2SO4 pH <2 +0-6°C 200mL
Phosphate, Ortho (as P); SM 4500P E Plastic / Glass 0-6°C 100mL
Phosphate, Ortho, Dissolved (as P); SM 4500P E Plastic / Glass 0-6°C 100mL
Phosphorus, Total (PO4as P); SM 4500P B/F or B/E 28 days Plastic / Glass H2SO4 pH <2+0-6°C 100mL
Solids, Settleable (SS); SM 2540 F Plastic / Glass 0-6°C 1000mL
Solids, Total (mg/L or %); SM 2540 B, or SM 2540 G 7 days Plastic / Glass 0-6°C 100mL
Solids, Total Dissolved (TDS); SM 2540 C 7 days Plastic / Glass 0-6°C 100mL
Solids, Total Suspended (TSS); SM 2540 D 7 days Plastic / Glass 0-6°C 200mL
Solids, Volatile Suspended (VSS); SM 2540 E 7 days Plastic / Glass 0-6°C 200mL
Sulfate (SO42-), EPA 300.0 28 days Plastic / Glass 0-6°C 100mL
Sulfide, Dissolved; SM 4500 S2- B/D 7 days Glass 250 mL AlCl3 + NaOH +0-6°C 250mL
Sulfide, Total; SM 4500 S2- C/D 7 days Glass 250 mL NaOH + ZnAC pH >9 + 0-6°C 250mL
Sulfite (SO3) 7 days Glass 500 mL EDTA + 0-6°C 250mL
Surfactants (MBAS); SM 5540 C Plastic / Glass 0-6°C 250mL
Suspended Sediment Conc. (SSC); ASTM D3977 7 days Plastic / Glass 0-6°C {7} 100mL
Total Organic Carbon (TOC); SM 5310 B 28 days Amber Glass VOA vial (in trip) HCl pH <2 + 0-6°C 40mL
Turbidity; SM 2130 B, or EPA 180.1 Plastic / Glass 0-6°C 100mL
Ultraviolet Absorption (UVA at 254nm); SM 5910 B Glass Amber 125mL 0-6°C 125mL

 

{1} Volatile organic samples need to be filled in multiple VOA vials without air bubbles or headspace.
{2} Dissolved metals require field or lab filtration through 0.45-micron filter prior to preservation. 40 CFR 136.3 requires filtration within 15 minutes.
{3} Volatile organic methods EPA 624 and 8260 require dechlorination using Sodium Thiosulfate (Na2S2O3) at time of sampling if chlorine is present. (Thiosulfate dechlorination bottles are available at the laboratory upon request.) Dechlorination is to occur before transferring to the appropriate VOA.
{4} Volatile organic method EPA 524.2 requires dechlorination using ascorbic acid at time of sampling if chlorine is present. (Ascorbic acid dechlorination kits are available at the laboratory upon request.) If analyzing for THMs only, Sodium Thiosulfate (Na2S2O3) may be used, and acidification can be omitted. Otherwise, dechlorinate with Ascorbic acid, then preserve with HCl. If the sample foams vigorously upon addition of HCl, discard and collect unpreserved dechlorinated sample, and notify the laboratory as the samples must be analyzed within 24 hours of collection if they are to be analyzed for any compounds other than THMs. Method 524.2 requires a travel/trip blank with each sample set collected.
{5} If sampling from a chlorinated location, collect additional bottles with 0.008% Sodium Thiosulfate (Na2S2O3) for dechlorination.
{6} Semi Volatile Organics Amber Liters (AL) should be collected in duplicate, to insure volume for re-extraction if necessary. When possible, please collect 4 AL per method to allow the laboratory to perform matrix Quality Control (MS/MSD).
{7} Suspended Sediment Conc.(SSC) requires its own container and the entire contents are used for the analysis.
{8} Preserve with HCl if less than 10 ppth Salinity, or Preserve with H2SO4 if greater than 10 ppth Salinity.
{9} Cyhalothrin in water has a 3 day hold time in reagent water per USGS study. Permethrin in water has a 3 day hold time per Storage Stability Study by CA Dept. of Food and Agriculture.
{10} 1000 mL required for RL of 5 mg/L. 250 mL bottle recommended for samples with expected concentrations of 20 mg/L or higher.
{11} If unpreserved, must be analyzed within 7 days of sampling
{12} Hold time can be extended to 14 days if preserved to pH 4-5 at time of collection.

Note: Multiple analyses may be run from the same container, provided that the bottle type, volume and preservation are appropriate.
(Rev 12.13.2018)

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